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Disk Map 2 5 X 5

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Cat. no. Z7031 X-Factor 1x50 disks/cartridge
Cat. no. Z7041 V-Factor 1x50 disks/cartridge
Cat. no. Z7051 XV-Factor 1x50 disks/cartridge
  1. Disk Map 2 5 X 5/8
  2. Disk Map 2 5 X 5 Rug

Disk Map 2.5 MAS macOS 7 mb. Disk Map - Visualize Hard Drive Usage & Free Space Disk Map makes it easy to visualize the files and folders that are cluttering up all your internal and external storage drives. It enables you to quickly locate, delete or compress large files and folders and reclaim tons of valuable space. Disk Map took exactly 3 minutes to scan 423.8 GB. DaisyDisk took 1 minute, 5 seconds in Administrator mode. So DD is certainly much faster, however I find the Disk Map results much easier to read, although I find them somewhat disarranged. These kits allow you to put front disc brakes on your 1948-56 Ford F100 1/2 ton pickup trucks. No spindle modifications are required - this is a bolt-on kit. This F100 disc brake conversion kit uses '76-'81 Dodge Aspen brake rotors (or Chrysler equivalent) and '70-'77 Camaro brake calipers (or GM equivalent) on stock '48-'56 Ford half ton pickup truck spindles. Rotors have a 5 on 4-1/2' Ford.

INTENDED USE

Hardy Diagnostics X - and V-Factor Disks are paper disks impregnated with X (hemin) and V (nicotinamide adenine dinucleotide - NAD) growth factors. They are used for the differentiation of Haemophilus species, including Aggregatibacter aphrophilus based upon their requirements for the growth factors.

SUMMARY

Haemophilus spp. have varying requirements for X and V growth factors. Consequently, the significant differences in growth factor requirements of Haemophilus spp. allows for their differentiation. Differentiation is based on the presence or absence of growth around and/or between disks impregnated with factors X, V and XV.

Use of paper disks impregnated with the growth factors was first proposed by Parker and Hoeprich.(4)

The determination that H. influenzae requires two growth factors was made by Davis, Thjotta, and Avery.(1,2)

FORMULA

Each X-Factor Disk is impregnated with hemin. Each V-Factor Disk is impregnated with NAD (nicotinamide adenine dinucleotide). Each XV-Factor Disk is impregnated with a combination of hemin and NAD.

STORAGE AND SHELF LIFE

Storage: Upon receipt, store at -20 to 8ºC. away from direct light. The disks should not be used if there are any signs of deterioration, discoloration, or if the expiration date has passed. Receipts 1 9 7 kilograms. Protect from light, excessive heat, and moisture.

The expiration date applies to the product in its intact packaging when stored as directed.

PRECAUTIONS

PROCEDURE

1. Using aseptic techniques, select isolated colonies of suspect colonies and prepare a suspension equivalent to a 0.5 McFarland standard (Cat. no. MS05). Tryptic Soy Broth (Cat. no. R30) or sterile Saline, 0.85% (Cat. no. K59) can be used for making suspensions.

Note: Due to the possibility of carryover of growth factors, do not cool the inoculating loop in the primary isolation medium before selecting colonies.

2. Using a sterile swab, inoculate the entire surface of a factor-free media such as Brain Heart Infusion Agar (Cat. no. W15). If Brain Heart Infusion Agar is unavailable, Mueller Hinton Agar (Cat. no. G45) or Tryptic Soy Agar (Cat. no. G60) may be substituted. Inoculate by streaking in three different directions by rotating the plate in a 60 degree angle after each streaking. Streaking in this manner ensures confluent growth.

3. Allow the surface to dry 3-5 minutes.

4. Using aseptic technique, apply disks around the periphery of the plate (approximately 1-2cm from the edge of the medium). Apply disks in the following positions:

X-Factor Disk 12 o'clock
V-Factor Disk 4 o'clock
XV-Factor Disk 8 o'clock

5. Incubate inoculated media 18-48 hours at 35ºC. in 5-10% CO2. Observe for growth or no growth around the disks.

INTERPRETATION OF RESULTS

Organisms that require only X-Factor will grow only in the area of the X- and XV-Factor Disks. Organisms that require only V-Factor, will grow only in the areas of the V- and the XV-Factor Disks. If both X- and V-Factors are required, the organism will grow only in the area of the XV-Factor Disk.

In cases where slight growth occurs around the V-Factor Disk, the growth must be at least equal in amount to that around the XV-Factor Disk before the organism can be considered H. parainfluenzae.

Haemophilus species Growth Around Disk
X V XV
Haemophilus aegyptius - - +
Haemophilus aphrophilus v/- - +
Haemophilus ducreyi + - +
Haemophilus haemolyticus - - +
Haemophilus influenzae - - +
Haemophilus parahaemolyticus - + +
Haemophilus parainfluenzae - + +
Aggregatibacter aphrophilus (formerly H. parainfluenzae, ATCC® 7901) - + +

LIMITATIONS OF THE PROCEDURE

Because similarities exist in growth factor requirements of Haemophilus species, it is not recommended that this procedure be the sole criterion for species identification.

Care should be taken during inoculation of specimens onto culture media in order to prevent nutrient carryover.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, slides, staining supplies, other culture media, microscopes, incinerators, and incubators, etc., as well as catalase, and other biochemical and serological reagents, are not provided.

Disk Map 2 5 X 5

QUALITY CONTROL

Expected Results
Test Organisms Reaction
Haemophilus influenzae
ATCC ® 10211
X-Factor: No Growth
V-Factor: No Growth
XV-Factor: Growth
Aggregatibacter aphrophilus (formerly Haemophilus parainfluenzae) ATCC ® 7901 X-Factor: No Growth
V-Factor: Growth
XV-Factor: Growth

User Quality Control

It is recommended that each lot or shipment of XV-Factor disks be checked with a positive control, and the X-Factor and V-Factor disks be tested with both known positive and negative controls.(6,7)

Showing growth of Haemophilus influenzae (ATCC® 10211) around the XV-Factor Disk (Cat. no. Z1051) only. This growth pattern demonstrated the inability of the organism to grow in the absense of either V-Factor or X-Factor and was consistant with H. influenzae. Incubated on Mueller Hinton Agar (Cat. no. G45) in CO2 for 48 hours at 35ºC.

Showing closer image of Haemophilus influenzae growth around the XV-Factor disk from the photo to the left.

REFERENCES

1. Davis, D.J. 1917. J. Infect. Dis.; 21:392.

2. Thjotta, T., and Avery, O.T. 1921. J. Exper. Med.; 34:97-114.

3. Lwoff, A., and Lwoff, M. 1937. Studies on codehydrogenosis, I.Proc. Roy. Soc. London Ser. B.; 122:352-359.

4. Parker, R.H., and Hoeprich, P.D. 1962. Am. J. Clin. Path.; 37:319-327.

5. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

6. Commission on Laboratory Accreditation, Laboratory Accreditation Program Microbiology Checklist. College of American Pathologists. Rev. 9/30/2004.

7. Centers for Medicare and Medicaid, Appendix C, Survey Procedures and Interpretive Guidelines for Laboratories and Laboratory Services. Subpart K - Quality System for Non-Waived Testing. 493;1200-1265. www.cms.hhs.gov/clia.


ATCC is a registered trademark of the American Type Culture Collection.

IFU-10851[A]


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Summary :

Does your disk appear as unknown or not initialized in Disk Management? This post talks about the two causes of this issue: unallocated space or no size. Keep reading to learn how to fix and recover data from a not initialized or unallocated disk.

Quick Navigation :

Disk Map 2 5 X 5/8

Hard Disk Shows 'Unknown Not Initialized' in Windows

'When I plug in the drive, it says that it's successfully installed however it does not appear in 'my computer'. Also when I go into system tools it says that disk 1 is unknown, not initialized, unallocated. Can you please help me, I don't want to lose my data, it contains all my pictures.'sevenforum

Like the case above, the issue 'disk unknown not initialized' happens not only on removable hard drives but also on internal or external hard drives from major manufacturers like Western Digital, Samsung, etc in Windows 10/8/7.

When it occurs, your hard drive may show unallocated space in Disk Management, and the data on which will be inaccessible. Breeze 1 64.

In addition, there is another situation: there is no disk space shown in Disk Management.

Tip: Sometimes you may find your external hard drive not showing up. What should you do? This post - 10 Situations Where External Hard Drive Not Showing up & Solutions shows you the fixes.

In general, a disk being unknown or uninitialized can be caused by various events including a virus attack, corrupted Master Boot Record, partition loss, bad sectors, and some computer file system corruption. If you are having trouble fixing your hard disk, please try the following solutions.

How to Solve Disk Unknown Not Initialized Windows 10/8/7

Case 1: Disk Unknown, Not initialized, Unallocated

First of all, open Disk Management and find the uninitialized disk. If its disk capacity is shown, that means your Windows operating system has recognized this disk, but its partition is lost or MBR is corrupted due to some reasons. In this case, you can use a professional software to fix the MBR and recover the partition.

Solution 1: Initialize Your Hard Drive

When it comes to hard disk not initialized, the first thing that you may do is to initialize this hard drive. Run Disk Management by right-clicking 'My computer' and then selecting 'Manage'. You can also press the keys 'Win + R' and input 'compmgmt.msc' to run the partition management tool.

Next, find the external hard drive connected to THIS computer. You will see this unknown disk appearing as 'unallocated'. If this external hard drive does not contain any important files, you can go ahead and initialize the disk without losing data. In Windows 10, initializing the disk to MBR or GPT should directly solve this problem.

Tip: What happens if I initialize my disk? Disk initialization can wipe out all the data and cause data loss. As a result, if you have stored a large amount of data on the external hard drive, an initialization operation like this is not recommended.

Therefore, due to the effects of data loss, this solution is not always the best.

Solution 2: Rebuild MBR

MBR, short for Master Boot Record, is a special type of boot sector at the beginning of the hard drive. It could possibly be corrupted due to many reasons, such as malware infection, accidental power failure, and mistaken operation.

If the MBR gets corrupted, the operating system will no longer know where to access its data. This is when the issue 'disk 1 unknown not initialized external hard drive' could occur. Therefore, you need to rebuild MBR for your hard disk to solve this issue.

How to fix MBR in Windows 10?

You might have heard about a highly popular tool for partition and disk management - MiniTool Partition Wizard.

This partition and disk manager is designed to do partition and disk management in different operating systems, including Windows Vista/XP/7/8/8.1/10, Windows Server 2003/2008/2012/2016.

It has multiple different editions - Free, Pro, Pro Deluxe, Pro Ultimate, Server, Enterprise, and Technician Edition - each having different functions. Click here to learn about their differences.

To fix a corrupted Master Boot Record, using the Free Edition would suffice. First, download this free tool and install it on your computer.

Step 1: Run MiniTool Partition Wizard Free Edition.

Step 2: In the left pane of the main interface, select the unknown external hard drive and click 'Rebuild MBR' from 'Check Disk' menu. You can also right-click the target disk and select this function.

Step 3: You will be prompted back to the main interface. Here, click 'Apply' to execute the change.

If this method also cannot fix the issue - disk 1 unknown not initialized Western Digital, try another solution as follows.

Solution 3: Partition Recovery

Rebuilding MBR cannot always fully work on the 'disk unknown not initialized' issue. In Disk Management, if you find disk shows unallocated space, just try to do partition recovery.

Note: Don't create a new partition on the unallocated disk, or it will be really difficult for partition recovery.

MiniTool Partition Wizard also provides a feature called 'Partition Recovery'. It helps you recover the lost partition as well as its data. This will be useful as long as the new partition has not been created.

Note that this is a paid feature. To use it, you can purchase its Pro Ultimate Edition.

Tip: Hard drives (both MBR and GPT) that have disk unknown not initialized issue will be shown as MBR disk in Partition Wizard. If it was a GPT disk and now is shown as MBR, please convert it to GPT before you recover partition. Otherwise, there will be a failure.

If you find the message 'hard drive not initialized in Disk Management', please try this solution in sevenforums using partition recovery to solve this issue. Here is a step-by-step guide below in Windows 10.

Step 1: Run MiniTool Partition Wizard. Then right-click on the target disk that has 'unallocated space', and select 'Partition Recovery'. Alternatively, click this function from the 'Check Disk' drop-down menu.

Note: It is likely that your external hard drive could appear as 'bad disk' in MiniTool Partition Wizard. In this case, you can also right-click on this disk to make a partition recovery.

Step 2: Select the scanning range for the specified disk. There are three scanning range as follows:

  • Full Disk scans the entire disk, which is very useful for finding the lost partition.
  • Unallocated Space helps to only scan free space.
  • Specified Range scans specified sectors. When clicking this option, you can change 'Starting LBA' and 'Ending LBA' according to your needs.

Choose one of the scanning range as you like, then click 'Next' to continue.

Step 3: Select a scanning method.

  1. Quick Scanrestores your partition if your lost/deleted partitions are continuous. When the program finds a lost/deleted partition, it will jump to the end of the partition to continue scanning other lost/deleted partitions.
  2. Full Scanwill scan each sector of the specified scanning range. If quick scan cannot find the lost partitions, try full scan.

Click 'Next' to continue doing partition recovery.

Step 4: Wait for this scan to complete finding the lost or deleted partitions. After finishing a scan, all lost partitions will be displayed in the following list. Please select the partitions you need.

Here you can double-click the searched partition to explore its content. After selecting your desired partitions, click 'Finish' to go back to the main interface.

Step 5: In the main interface, you can preview all lost partitions. Click 'Apply' to execute all changes.

Tip: This screenshot shows you the partitions you will recover, which have not been assigned drive letters. You need to use MiniTool Partition Wizard to change drive letter.

Get MiniTool Partition Wizard now to try fixing the hard drive not initialized in Disk Management issue. If you find it useful, share it with other people to help them too.

After performing partition recovery, the issue of disk 1 unknown not initialized external hard drive should be fixed, and all data on this disk should be able to be accessed.

Solution 4: Recover Data from Unknown Disk

What should do if you have a large amount of important data saved on a hard drive with lost or deleted partitions, and 'Partition Recovery' can't help you?

Don't panic! The data on your external hard drive can be recovered by MiniTool. It offers a 'Data Recovery' feature that quickly recovers data when a hard drive is not initialized with simple clicks. Try it with MiniTool Partition Wizard Pro Ultimate.

Step 1: Choose the unknown not initialized disk and click the 'Data Recovery' feature from 'Check Disk'.

Step 2: This software will start scanning the selected disk; wait patiently until the scan completes.

Step 3: After the scan is complete, you can check the files that you need from the list it shows you. Then choose these items and click 'Save' to recover them to a safe location.

Related article:Recover Data from Disk Shows as Unknown without Damaging It

For more information about case 1, you can watch the following video.

Case 2: Disk Unknown Not Initialized No size in Disk Management

If Disk Management is unable to show any information on neither this hard drive nor its disk capacity, the hard drive might have some disk problem or cartridge problem. Performing operations might be blocked by the 'external hard disk not initialized I/O' error.

Solution 1: Turn off PC and Remove Battery

Perhaps disk unknown not initialized no size issue is caused by the PC motherboard issue. Just try to remove the battery to fix the issue:

  1. Turn off the computer.
  2. Disconnect the data and power cables from the problematic drive.
  3. Remove the CMOS battery for ten more seconds.
  4. Insert the CMOS battery.
  5. Turn on the computer.
  6. Shut down your computer.
  7. Connect data and power cable of the problematic drive.
  8. Start the computer.

If this method doesn't work, please continue reading.

Solution 2: Replace the Bad External Hard Drive

Usually, Disk Management is unable to grab the disk information or the capacity of a disk that is unknown or not initialized. This issue could still exist even when you connect the disk to another computer.

In this case, you should take this disk out and send it to professionals for an inspection. If there is a physical error, the only solution to the unknown not initialized no size issue is to throw it away and replace this bad external hard drive.

In addition, there are still some disk problems in Disk Management, such as hard disk is not detected and hard drive space is missing, the following two articles could be useful.

Conclusion

In summary, this issue 'disk unknown not initialized' can be solved quickly and easily with the assistance of MiniTool Partition Wizard. When addressing this problem, please try MiniTool products to rebuild MBR, recover partition and retrieve data. Hope you find this post useful.

Please leave a comment below or contact [email protected] if you have any suggestion or question. Thanks in advance!

Disk Unknown Not Initialized FAQ

???? How do I fix disk 1 unknown is not initialized?
When you encounter the issue of disk unknown not initialized in Windows 10/8/7, you can try multiple solutions to fix it, for example, initialize your hard drive, rebuild MBR, perform partition recovery or recover data from unknown disk, turn off your PC and remove the battery and replace the bad hard drive.
Of course, initializing a disk will erase it. That is, when you initialize a hard drive, you make it usable by Windows operating system but all the contents are removed.
Disk initialization can wipe out all the disk data and cause data loss. If there is a large amount of data on your hard drive, it is not recommended to initialize a disk.

Disk Map 2 5 X 5 Rug

In Windows 10/8/7, press Win +R to open the Run dialog, input diskmgmt.msc and click OK to open Disk Management. Right-click your target hard drive that you need to initialize, choose Initialize Disk and choose a partition style.




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